... Testing Other tests have included immunofluorescence and radio- immunoprecipitation assays, but the most extensive experience has been ...

... 1.5 mm) from VWR Scientific. Metabolic labeling, immunoprecipitation, and subcellular fractionation. COS7 cells were labeled with ... of fraction V bovine serum albumin per ml). Immunoprecipitations with polyclonal rabbit sera and mono- clonal antibodies ...

... virus, washed, and lysed with detergent buffer; and immunoprecipitations were performed on the lysates (Fig. la). Antibodies ... with virus; lane 3, nonimmune human IgG precipitate. Immunoprecipitations with antibody to HITLV-IIVLAV were also performed ...

... used. These include polyacrylamide and agarose gel electrophoresis, immunoprecipitation, Western blotting and screening, column chromatography, isoelectric focusing, ...

... antibodies present in the two patients’ sera, several immunoprecipitation ex- periments were carried out. Cord lym- - phocytes ... we — |= aay eT ow —_ — . -_ ad " 46. Fig. 3. Immunoprecipitation of ?’S-labeled viral proteins. Cord blood T-lymphocytes ...

... Translation—JIn vitro translation of CDNA transcripts and immunoprecipitation were carried out as described (40). Comparison of ... vitro translation products of transcripts of Nup475 after immunoprecipitation. Lane J, protein markers (kDa); lane 2, antiserum ...

... rabbits immunized with tobacco smoke components reacted by immunoprecipitation with tobacco smoke or leaf antigens (62). These ...

... lines may appear that do not reflect true immunoprecipitation. Puri- fied agar and especially sulfate-free agar ( ... do not diffuse freely, usually give a sharp immunoprecipitation line with anti-6-lipoprotein serums (Fig. 2). ...

... Testing Other tests have included immunofluorescence and radio- immunoprecipitation assays. but the most extensive experience has been ... a Other tests have included immunofluorescence and radio- immunoprecipitation assays. but the most extensive experience has been ...

... chromatography, DEAE-cellulose chromato- graphy, CMC-cellulose chromatography, immunoprecipitation, gel electrophoresis, cesium chloride density gradient centrifugation, as ...