... III holoenzyme, and primase were included. Other Methods. Agarose gel electrophoresis of DNA was as described (16, 19). ... of primase abolished almost 80% of this activity. Agarose gel electrophoresis of the G4 DNA prod- ucts showed ...

... 1975 Electrophoretic Separation of Bacillus subtilis Genes (EcoR1/agarose gel electrophoresis/genetic transformation /DNA/restriction nuclease) R. M. ... 3 kilobases and can be partially separated by agarose gel electrophoresis. Various markers can thus be enriched from ...

... extraction (Hirt, 1967) and electrophoresis in 1.4% agarose gel slabs, as described elsewhere (Brock- man and Nathans, ... was then subjected to electrophoresis in 1.4% agarose gel slabs to separate mutant from helper virus DNA, ...

... A protein-RF II complex under these conditions. Agarose Gel Electrophoresis. For analysis of DNA products, the reaction ... 12). The circles, identified autoradiographically after separation by agarose gel electro- phoresis (Fig. 2), were the exclusive product ...

... infected cell lysates. Such short genomes, isolated by agarose gel electrophoresis, were shown to be specifically altered by ... AZethods, and subjected the DNA to electrophoresis in agarose gel slabs. As illustrated in Fig. 1, among a ...

... electrophoretically analyzed on gradient polyacrylamide slab gels or agarose gels, and the three viruses were found to have ... for the H. influenzae digestion mixture) or on agarose gel (for the H. parainfluenzae and E. coli digestion ...

... ml) and 2 volumes of ethanol. Gel Electrophoresis. Agarose gels (1.2%, 6 X 200 mm) were prepared ... 4 ug of DNA were applied to each agarose gel; elec- trophoresis was for seven hours at 60 ...

... sample was subjected to electrophoresis in 1.4% agarose gel as described previously (Brockman and Nathans, 1974). In ... stocks were surveyed in two ways: 1) By agarose-gel electrophoresis to detect short genomes, and 2) by ...

... and Kornberg out in 0.7-3.5% agarose gels at constant voltage (1 V/cm) at room ... al. (23). To isolate DNA frag- ments from agarose gels, the gels were digested with 1.5 vol ...

... Filter Hybridization. RNA was electrophoresed on denaturing formaldehyde agarose gels (32, 33) and transferred to nitrocellulose (34). Filters ... 1.0 * Insert sizes were measured on 2% agarose gels by comparing the mo- bilities of DNA fragments ...