... L.Agar Acid Inert Alkal. Count B.E.Agar Dilution 24 48 72 Plate Stab. Tndol Nitrate Gelatine ... L.Agar Acid Inert Alkal. Count B.E.Agar Dilution 24 48 72 Plate Stab. Indol Nitrate Gelatine ...

... intervals during incubation was determined by the usual agar dilution plates and colony counts. In this work, however, ... made in a beef broth medium. Zero- time dilution cultures are made on agar plates. Samples from MONOCHROMATIC ULTRAVIOLET RADIATION 33 the ( ...

... used. Esmarch's Roll-tubes.-Use liquefied gelatin or agar. The dilutions in tubes 1, 2 and 3 are made ... another, the " dilution-flask." After two minutes, three agar tubes are inoculated with 1 c.c. each from the dilution-flask, plated, and growth watched for. Control-experiments ...

... was obtained by plating suitable dilutions on nutrient agar. Various dilutions of cells were plated with a constant volume ... and cells (LA-22) were mixed at various dilutions and plated on minimal agar. Prototrophs were counted after 48 hours. amount of ...

... mixed culture are made in tubes of melted agar. Usually three dilutions are sufficient to obtain isolated colonies. One or ... incubate. e. Pour a plate of nothing but agar, and f. Pour a plate using 1 cc, of water which was used for the dilutions, g. These plates are controls. 3. Incubation: plates ...

... inoculated cultures are subcultured to two azide-blood agar plates in serial dilution, as well as to a tube of glycerol ...

... 8, and 10 are presented. plated on blood agar after appropriate dilution. The plates were incubated 2 hr at 37°C, to allow expression of phenotype, overlayered with an equal volume of antbiotic-containing agar, to select the transformants, and colonies were counted ...

... developed either by the use of the double-agar or limiting dilution technique. These clones or lines are then tested ...

... variation of this technique, known as the rocker-dilution agar technique, is also useful when one desires to ...

... hour. 2. Prepare a twenty-four-hour-old agar culture of a virulent strain of Bacillus cholera. 3. Prepare a 1 :100 dilution of the immune serum and place 3 c. ...