... according to the method of Kalckar (5), and 5/-nucleotidase was obtained from bull semen according to Heppel ... by the liberation of inorganic phosphate (8) with 5’-nucleotidase (6), while acetate was measured with a partially ...

... described in the previous paper (1). Bull semen 5/-nucleotidase was prepared according to the method of Heppel ... phosphomonoesterase.......... 0.66 0.39 P; after phosphodiesterase + 5’-nucleotidase..| 1,27 0.89 P; after phosphodiesterase + semen ...

... phosphodiesterase, specific for single stranded DNA, and specific 5’-nucleotidase from Crotalus adamanieus venom were purified and assayed ... the DNA phosphatase activity (1); for susceptibility to 5’-nucleotidase, a 0.05-ml aliquot was assayed in ...

... 23); E. coli alkaline phosphatase, Worthington Biochemical Corporation; 5’-nucleotidase, Lehman, Roussos, and Pratt (24); and micrococcal nuclease, ... DNA with either bac- terial alkaline phosphatase or 5’-nucleotidase was measured as Norit-nonadsorbable material. Release of # ...

... cyclic nucleotide phosphodiesterase in rod outer segments, (2) 5 ' -nucleotidase that regulates 5 ' -GMP level of rod outer ... metarhodopsin I. III. There are two types of 5 '-nucleotidase in rod outer segments: membrane bound (integral) enzyme ...

... to catalyze. 11. Your comment that resistance to 5'-nucleotidase is not a proof of structure is quite ... activity which made a 5'-deoxynucleotide insensitive to 5'-nucleotidase. We found that the only significant reaction, even ...

... by seven [?] are but out[?] by bull semen 5-nucleotidase and they have the right PF in 2 ... tide phospholase[?] and then adding GDP ase and 5-nucleotidase, forming gerasine[?] from residual GDP and GMP. It ...

... using bovine serum albumin as standard. “Marker” Enzymes—5'-Nucleotidase (EC 3.1.3.5) was measured by ... min/mg protein wmoles/10 min/mg protein 5'-Nucleotidase.................. 0.032 0.535 0.632 None 0. ...

... alkaline phosphatase, glucose-6-phosphatase, adenosine triphosphatase, and 5-nucleotidase, among others-~are inhibited by beryllium salts?-7>’ ... activity, phosphoglucomutase activity but not Peer neers and 5-nucleotidase activity. Varma and Srinwasan reported that Be inhibited ...

... a UDPGase). Also, a specific protein inhibitor for 5'-nucleotidase and UDPGase has been found; its role in ...